A review of reaction enhancement strategies for isothermal nucleic acid amplification reactions

Isothermal nucleic acid amplification techniques are used to detect a variety of molecules such as RNAs, DNAs and proteins. When compared the gold standard polymerase chain reaction, isothermal methods rapid simple but often have lower specificity sensitivity. In this review, we provide summary reaction additives that enhance sensitivity, specificity, stability, robustness, or speed reactions for sensing applications. We also mechanism purpose each enhancement technique, which can guidance when selecting optimize novel systems assays.